US8670942B2 - Flow cytometer remote monitoring system - Google Patents
Flow cytometer remote monitoring system Download PDFInfo
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- US8670942B2 US8670942B2 US12/736,684 US73668409A US8670942B2 US 8670942 B2 US8670942 B2 US 8670942B2 US 73668409 A US73668409 A US 73668409A US 8670942 B2 US8670942 B2 US 8670942B2
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- data representation
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- byte data
- flow cytometer
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Classifications
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04L—TRANSMISSION OF DIGITAL INFORMATION, e.g. TELEGRAPHIC COMMUNICATION
- H04L67/00—Network arrangements or protocols for supporting network services or applications
- H04L67/01—Protocols
- H04L67/06—Protocols specially adapted for file transfer, e.g. file transfer protocol [FTP]
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1425—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its control arrangement
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00722—Communications; Identification
- G01N35/00871—Communications between instruments or with remote terminals
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04L—TRANSMISSION OF DIGITAL INFORMATION, e.g. TELEGRAPHIC COMMUNICATION
- H04L67/00—Network arrangements or protocols for supporting network services or applications
- H04L67/01—Protocols
- H04L67/12—Protocols specially adapted for proprietary or special-purpose networking environments, e.g. medical networks, sensor networks, networks in vehicles or remote metering networks
-
- G01N15/1433—
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G01N15/149—
-
- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05B—CONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS
- G05B2223/00—Indexing scheme associated with group G05B23/00
- G05B2223/06—Remote monitoring
Definitions
- One substantial problem with using a substantial portion of the history and detail of viewable data representation may be competition for computer processing capacity of the flow cytometer device (or other devices which provide similar viewable data representations) resulting in a delay updating display of the viewable data representation which in certain instances can appear as interrupted or non-continuous display of the viewable data representation.
- attempts to access and utilize stored viewable data representations or attempts to control the functionalities of the flow cytometer device remotely can interfere with the normal operation and particle analysis of the flow cytometer device.
- FIG. 5 is block diagram of hardware means which can be utilized in a particular embodiment of the invention.
- FIG. 7 is a block diagram which shows a particular method of archiving reduced byte data representations generated by a particular embodiment of the invention.
- inventive instrument or flow cytometer remote monitoring system ( 1 ) may be described herein in terms of functional block components, screen shots, and various process steps. It should be appreciated that such functional blocks may be realized by any number of hardware or software components configured to perform the specified functions.
- inventive flow cytometer remote monitoring system ( 1 ) may employ various integrated circuit components which function without limitation as memory elements, processing elements, logic elements, look-up tables, or the like, which may carry out a variety of functions under the control of one or more microprocessors or other control devices.
- Each interrogated one of the plurality of particles ( 5 ) can in response to interrogation by the laser beam(s) absorb or emit an amount of light ( 12 A).
- DNA can be quantitatively stained with a dye or fluorochrome such as Hoechst 33342 .
- the stained DNA can emit an amount of light ( 12 ) in response to being interrogated with a laser beam.
- X chromosome bearing sperm cells ( 14 ) typically emit a greater amount of light ( 12 A) than Y chromosome bearing sperm cells ( 15 ) because each X chromosome bearing sperm cell ( 14 ) contains a greater amount of stained DNA than a Y chromosome bearing sperm cell ( 15 ).
- a plurality of particle populations ( 30 ) can include X chromosome bearing sperm cells ( 14 ) isolated in the first container ( 32 ) and Y chromosome bearing sperm cells ( 15 ) isolated in the second container ( 33 ).
- a reduced byte data representation files comprises an image file of a lesser bytes than the corresponding time bound data representation file.
- each of the plurality of reduced byte data representation files will comprise between about one hundred kilobytes and about two hundred kilobytes (although the invention is not so limited and each file can include a lesser or greater number of bytes).
- the reduced byte data representation file ( 39 ) can be in the form of a .jpeg. which includes substantially lesser bytes than for example a Windows Bitmap format which can be one form of the plurality of time bound image representation files ( 35 ) transferred from the first computer ( 16 ) to the second computer ( 37 ).
Abstract
Description
Claims (36)
Priority Applications (2)
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US12/736,684 US8670942B2 (en) | 2008-05-02 | 2009-05-01 | Flow cytometer remote monitoring system |
US14/176,508 US10425466B2 (en) | 2008-05-02 | 2014-02-10 | Flow cytometer remote monitoring system |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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US12/151,156 US8060353B2 (en) | 2008-05-02 | 2008-05-02 | Flow cytometer remote monitoring system |
PCT/US2009/002715 WO2009134442A1 (en) | 2008-05-02 | 2009-05-01 | Flow cytometer remote monitoring system |
US12/736,684 US8670942B2 (en) | 2008-05-02 | 2009-05-01 | Flow cytometer remote monitoring system |
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US12/151,156 Continuation-In-Part US8060353B2 (en) | 2008-05-02 | 2008-05-02 | Flow cytometer remote monitoring system |
PCT/US2009/002715 A-371-Of-International WO2009134442A1 (en) | 2008-05-02 | 2009-05-01 | Flow cytometer remote monitoring system |
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US14/176,508 Continuation US10425466B2 (en) | 2008-05-02 | 2014-02-10 | Flow cytometer remote monitoring system |
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US20110054798A1 US20110054798A1 (en) | 2011-03-03 |
US8670942B2 true US8670942B2 (en) | 2014-03-11 |
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US12/736,684 Active 2029-12-22 US8670942B2 (en) | 2008-05-02 | 2009-05-01 | Flow cytometer remote monitoring system |
US14/176,508 Active 2030-08-20 US10425466B2 (en) | 2008-05-02 | 2014-02-10 | Flow cytometer remote monitoring system |
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